Journal: Diabetologia

Article Title: Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans

doi: 10.1007/s00125-011-2201-x

Figure Lengend Snippet: In vitro three-dimensional HUVEC angiogenesis model. Cells were treated with leptin (50 and 100 ng/ml, c and d) and VEGF (20 ng/ml as positive control, b) for 48 h. a The negative control. e Sprout counts; f sprout cumulative length. aNo statistical significance at the 0.05 level. bBox plot are statistically different from those marked with a. Statistical outliers are denoted with black circles

Article Snippet: In their seminal publication, Sierra-Honigmann et al. demonstrate that the Ob-Rb leptin receptor is also produced in human vasculature endothelial cells, suggesting that leptin could induce angiogenesis [ 7 ].

Techniques: In Vitro, Positive Control, Negative Control

Journal: Diabetologia

Article Title: Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans

doi: 10.1007/s00125-011-2201-x

Figure Lengend Snippet: Circulating angiogenic factor concentrations: short-term clinical study

Article Snippet: In their seminal publication, Sierra-Honigmann et al. demonstrate that the Ob-Rb leptin receptor is also produced in human vasculature endothelial cells, suggesting that leptin could induce angiogenesis [ 7 ].

Techniques:

Journal: Diabetologia

Article Title: Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans

doi: 10.1007/s00125-011-2201-x

Figure Lengend Snippet: Circulating angiogenic factor concentrations: long-term clinical study, metreleptin ( n =10), placebo ( n =8)

Article Snippet: In their seminal publication, Sierra-Honigmann et al. demonstrate that the Ob-Rb leptin receptor is also produced in human vasculature endothelial cells, suggesting that leptin could induce angiogenesis [ 7 ].

Techniques:

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Notch-1 decreased expression contributes to leptin receptor downregulation in nasal epithelium from allergic turbinates.

doi: 10.1016/j.bbadis.2019.03.016

Figure Lengend Snippet: Fig. 1. A. Immunohistochemistry for leptin receptor, leptin and TGF-β1,2,3 in epithelia from inferior turbinate biopsy specimens (red). Healthy subjects (C) and patients with allergic rhinitis (AR) are shown (magnification at X1000, scale bar = 40 μm). B, Leptin receptor and leptin expression are significantly lower* in AR (P = .003 and P = .006 respectively) versus the C group whereas TGF-β is not. The results are shown as box-plots with medians (lines inside the boxes), 25th and 75th percentiles (limits of boxes), and the 10th and 90th percentiles (whiskers). NS, not significant. Mann-Whitney U test analysis.

Article Snippet: The polyclonal anti-leptin receptor antibody (Ob-R M-18, sc-1834), against the common part of the short and long forms of leptin receptor, and the anti-leptin antibody (Ob A-20, sc-842), the DAPT peptide blocking Notch-1 signaling (LY-374973, sc-201,315), were from Santa Cruz Biotechnology.

Techniques: Immunohistochemistry, Expressing, MANN-WHITNEY

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Notch-1 decreased expression contributes to leptin receptor downregulation in nasal epithelium from allergic turbinates.

doi: 10.1016/j.bbadis.2019.03.016

Figure Lengend Snippet: Fig. 2. Immunofluorescence. On epithelium from inferior turbinate biopsy specimens, leptin/leptin receptor and TGF-β1,2,3 expressions are not co-localized. Leptin and its receptor appear more basolateral whereas TGF-β appears more apical (magnification at X400, Scale bar = 40 μm). LEICA DM 4000 microscope.

Article Snippet: The polyclonal anti-leptin receptor antibody (Ob-R M-18, sc-1834), against the common part of the short and long forms of leptin receptor, and the anti-leptin antibody (Ob A-20, sc-842), the DAPT peptide blocking Notch-1 signaling (LY-374973, sc-201,315), were from Santa Cruz Biotechnology.

Techniques: Microscopy

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Notch-1 decreased expression contributes to leptin receptor downregulation in nasal epithelium from allergic turbinates.

doi: 10.1016/j.bbadis.2019.03.016

Figure Lengend Snippet: Fig. 3. Immunofluorescence. On epithelium from inferior turbinate biopsy specimens, leptin receptor (green) and leptin (red) are readily detected (magnification at X400, Scale bar = 40 μm) and can be seen to co-localize. Leptin/leptin receptor levels appear diminished in AR as compared to C. LEICA DM 4000 microscope.

Article Snippet: The polyclonal anti-leptin receptor antibody (Ob-R M-18, sc-1834), against the common part of the short and long forms of leptin receptor, and the anti-leptin antibody (Ob A-20, sc-842), the DAPT peptide blocking Notch-1 signaling (LY-374973, sc-201,315), were from Santa Cruz Biotechnology.

Techniques: Microscopy

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Notch-1 decreased expression contributes to leptin receptor downregulation in nasal epithelium from allergic turbinates.

doi: 10.1016/j.bbadis.2019.03.016

Figure Lengend Snippet: Fig. 4. A. Correlations between expression of leptin receptor and leptin. In epithelia from all inferior turbinates leptin and leptin receptor expression are positively correlated with each other (Rho = 0.38; P = .019). Spearman's rank correlations. B. Quantitative real-time PCR of the leptin receptor transcripts is significantly reduced* in AR compared to C (P = .04). Mann-Whitney U test analysis. ΔCT is inversely correlated with the gene expression.

Article Snippet: The polyclonal anti-leptin receptor antibody (Ob-R M-18, sc-1834), against the common part of the short and long forms of leptin receptor, and the anti-leptin antibody (Ob A-20, sc-842), the DAPT peptide blocking Notch-1 signaling (LY-374973, sc-201,315), were from Santa Cruz Biotechnology.

Techniques: Expressing, Real-time Polymerase Chain Reaction, MANN-WHITNEY, Gene Expression

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Notch-1 decreased expression contributes to leptin receptor downregulation in nasal epithelium from allergic turbinates.

doi: 10.1016/j.bbadis.2019.03.016

Figure Lengend Snippet: Fig. 5. Immunofluorescence. On epithelium from inferior turbinate biopsy specimens, leptin receptor (green) and Notch-1 (red) are readily detected (magnification at X400, scale bar = 40 μm) and appear to be co-localized. Both markers appear diminished in AR relative to C. LEICA DM 4000 microscope.

Article Snippet: The polyclonal anti-leptin receptor antibody (Ob-R M-18, sc-1834), against the common part of the short and long forms of leptin receptor, and the anti-leptin antibody (Ob A-20, sc-842), the DAPT peptide blocking Notch-1 signaling (LY-374973, sc-201,315), were from Santa Cruz Biotechnology.

Techniques: Microscopy

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Notch-1 decreased expression contributes to leptin receptor downregulation in nasal epithelium from allergic turbinates.

doi: 10.1016/j.bbadis.2019.03.016

Figure Lengend Snippet: Fig. 6. A. Immunofluorescence for leptin receptor (green) and Notch-1 (red) on primary epithelium obtained from inferior turbinate biopsies from healthy subjects (C, n = 4) and from patients with allergic rhinitis (AR, n = 5) are shown (magnification at X630, Scale bar = 10 μm). Leptin receptor and Notch-1 in EPCAM positive cells appear decreased in AR, but not in C. Axioskop-2-Zeiss microscope. B, Leptin receptor and Notch-1 expression levels in primary turbinate epithelia (EPCAM positive cells) are significantly lower* in AR (P = .03 and P = .04 respectively) than in the C group. Furthermore, the leptin receptor expression in total cell extracts is significantly lower** in AR (P = .001) than in C. NS, not significant. Analysis of variance (ANOVA), Fisher's PLSD for log data. Right, Representative examples of flow cytometric analysis. The numbers indicate the percentage of double positive cells for leptin receptor/EPCAM, and for Notch-1/EPCAM, respectively.

Article Snippet: The polyclonal anti-leptin receptor antibody (Ob-R M-18, sc-1834), against the common part of the short and long forms of leptin receptor, and the anti-leptin antibody (Ob A-20, sc-842), the DAPT peptide blocking Notch-1 signaling (LY-374973, sc-201,315), were from Santa Cruz Biotechnology.

Techniques: Microscopy, Expressing

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Notch-1 decreased expression contributes to leptin receptor downregulation in nasal epithelium from allergic turbinates.

doi: 10.1016/j.bbadis.2019.03.016

Figure Lengend Snippet: Fig. 7. In the RPMI 2650 cell line, the expression of leptin receptor is significantly increased* (P = .005) by Notch-1 ligand (JAGGED-1, n = 14). Blocking Notch signaling (DAPT, n = 14) restored basal expression of leptin receptor expression and elicited a significantly reduced association ** (P = .02) in comparison to JAGGED-1 alone. The results are shown as box-plots with medians (lines inside the boxes), 25th and 75th percentiles (limits of boxes), and the 10th and 90th percentiles (whiskers). Analysis of variance (ANOVA), Fisher's PLSD for log data. Upper, Representative examples of flow cytometric analysis. The numbers indicate the percentage of leptin receptor positive cells. FSC-H, forward light scatter-height.

Article Snippet: The polyclonal anti-leptin receptor antibody (Ob-R M-18, sc-1834), against the common part of the short and long forms of leptin receptor, and the anti-leptin antibody (Ob A-20, sc-842), the DAPT peptide blocking Notch-1 signaling (LY-374973, sc-201,315), were from Santa Cruz Biotechnology.

Techniques: Expressing, Blocking Assay, Comparison

Journal: Nature Communications

Article Title: HGK/MAP4K4 deficiency induces TRAF2 stabilization and Th17 differentiation leading to insulin resistance

doi: 10.1038/ncomms5602

Figure Lengend Snippet: ( a , b ) CD4 + CD25 − T cells were purified from spleens of WT mice and cultured for 4 days under Th17 differentiation conditions in the presence or absence adipose tissue fluids ( a ) or 3T3-L1 adipocyte conditioned medium ( b ). After Th17 differentiation, T cells were stimulated with PMA and ionomycin, and analysed for IL-17 expression by intracellular staining. Contour plots depict IL-17-producing CD4 T cells. Numbers indicate the percentages of cells in the quadrants. ( c ) The levels of indicated adipokines from adipose tissue of WT and T-HGK cKO mice were determined by ELISA assays. ( d ) The IL-17 levels in supernatants of the primary T cells stimulated with leptin and/or IL-6 for 3 days were determined by ELISA assays. ( e ) IL-6 and IL-17 levels in the sera of 16-week-old T-HGK cKO (CD4-Cre;HGK f/f ) mice and T-HGK/LepR cKO (CD4-Cre;HGK f/f ;leptin receptor f/f ) mice were measured by ELISA assays. n =6. ( f ) GTT tests were performed on 16-week-old T-HGK cKO mice and T-HGK/LepR cKO mice. Glucose levels were plotted versus times post glucose injection. ( g ) The leptin levels in adipose tissue of mice after IL-6 neutralization. Data shown are representatives of three ( a , b , d ) and two ( c , e , f , g ) independent experiments. Data are expressed as mean±s.e.m. * P -value<0.05; ** P -value<0.01; *** P -value<0.001 (two-tailed Mann–Whitney U -test).

Article Snippet: Floxed leptin receptor mice (JAX008327) and CCR6 knockout mice (JAX013061) were purchased from the Jackson Laboratory.

Techniques: Purification, Cell Culture, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Injection, Neutralization, Two Tailed Test, MANN-WHITNEY

Journal: The Journal of Neuroscience

Article Title: Spatially Heterogeneous Choroid Plexus Transcriptomes Encode Positional Identity and Contribute to Regional CSF Production

doi: 10.1523/JNEUROSCI.3081-14.2015

Figure Lengend Snippet: qRT-PCR measurement of genes detected by RNA-seq to be differentially expressed between tChP and hChP

Article Snippet: Lepr , Mm00440181_m1 , 1.000 , 0.473 ± 0.088 , 0.012.

Techniques: